Miniature Dielectric Barrier Discharge Nonthermal Plasma Induces Apoptosis in Lung Cancer Cells and Inhibits Cell Migration

Figure 7

Viability and apoptosis analysis of 0 (no treatment), 15, 30, 60, and 120 sec samples using Annexin V-FITC and PI after 24 h of plasma treatment. (a) Viability percentage of cancer cells treated with plasma for four different times after 24 h of plasma treatment. (b) The percentage of cells in the four different quadrants. Upper-left: PI⁺ and Annexin V^-, necrotic cells; upper-right: PI⁺ and Annexin V⁺, late apoptotic cells; lower-right: Annexin V⁺ and PI^-, apoptotic cells; lower-left: PI^- and Annexin V^-, live cells. (c) Quantitative depiction of cell sorting data. Data are expressed as mean of cell number ± SD (). , , , and denote statistically significant differences between control and 15, 30, 60, and 120 sec plasma treatments, respectively ().

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