Collagen Sponge Functionalized with Chimeric Anti-BMP-2 Monoclonal Antibody Mediates Repair of Critical-Size Mandibular Continuity Defects in a Nonhuman Primate Model

Figure 5

Histologic and histomorphometric analysis of treated mandibular defects. Histomicrographs of H&E stained sections obtained from axial planes taken at the midpoint between superior and inferior boundaries of mandibular defects (a–j). Mandibular segmental osteotomy defects were treated with chimeric anti-BMP-2 mAb (a–e) or isotype-matched control mAb (f–j) immobilized on absorbable collagen sponge scaffold. Histological evaluation 12 weeks after surgery revealed the presence of mature lamellar bone in the newly ossified tissue of experimental defect treated with chimeric anti-BMP-2 mAb (a–e), whereas isotype-matched control mAb (f–j) was primarily filled with connective tissue. There was no evidence of inflammatory infiltration in either group. Black and green arrowheads represent osteoid bone or all other tissues (referred to as void), respectively. Quantitative histomorphometric analysis of new bone formation (k) confirmed higher degree of bone formation within defects treated with chimeric anti-BMP-2 mAb compared with isotype-matched control mAb ().

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