Figure 1: Western blot analysis of endogenously expressed FTO protein in 3T3-L1 (Lanes #1, #3, #5, and #7) and the WT FTO transfected SH-SY5Y control cells (Lanes #2, #4, #6, and #8). For detection of FTO and actin bands, mouse monoclonal anti-FTO and anti-actin antibodies were used, respectively. The FTO band was only observed in the WT FTO transfected SH-SY5Y cells. The lack of FTO band in 3T3-L1 cells implies the absence or low level of FTO expression.