Figure 2: SDS-PAGE analysis of the cell-free extracts prepared from the pcDNA4/TO, pcDNA4/TO-WT FTO and pcDNA4/TO-R316Q FTO transfected cells to demonstrate the moderate expression level of the FTO (a). Western blot analysis of the WT FTO and the mutant (R316Q) expressions in 3T3-L1 cells. The positive control was from the WT FTO transfected SH-SY5Y cell-free extract. The bar graph was created using the intensity values of the bands obtained from Image J.