Exogenous Expressions of FTO Wild-Type and R316Q Mutant Proteins Caused an Increase in HNRPK Levels in 3T3-L1 Cells as Demonstrated by DIGE Analysis

Figure 4

Representative 2D images of the DIGE gels (a). The green color represents Cy3 labeled protein extracts prepared from the WT FTO expressing cells. The red color represents Cy2 labeled protein extracts prepared from the mutant (R316Q) FTO expressing cells. The blue color represents Cy5 labeled protein extracts prepared from the pcDNA4/TO expressing cells. The Image for the 2D preparative gel (b). The spots labeled with SSP numbers were excised and identified.

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