Neutrophil Extracellular Traps Are Pathogenic in Ventilator-Induced Lung Injury and Partially Dependent on TLR4

Figure 6

Toll-like receptor 4 knockout prevents the development of lung injury (A). (a) The level of HMGB1 in BALF was significantly higher than that in TLR4 WT CON group and LTV group. (b) The lung wet/dry ratio in TLR4 KO HTV mice was decreased compared with the TLR4 WT mice following HTV. (c)(d) The TLR4 KO HTV mice showed a marked elevation in protein content and neutrophil counts in the BALF compared to the TLR4 WT HTV mice. (e)(f) The BALF levels of IL-6 and TNF-α in the TLR4 KO HTV mice did not increase as much as in the TLR4 WT HTV mice. From (b) to (f), two-way ANOVA indicated both TLR4 genotype (TLR4 WT versus KO) and ventilation were significant, but there was no interaction (). .

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