Neutrophil Extracellular Traps Are Pathogenic in Ventilator-Induced Lung Injury and Partially Dependent on TLR4

Figure 8

Toll-like receptor 4 contributes to the NET formation in VILI in mice. (a) In contrast to the TLR4 WT mice, HTV partially increased Cit-H3 expression in the lung homogenates in the TLR4 KO mice. (b) The ratio of the Cit-H3/GAPDH intensity in the TLR4 KO HTV group was lower compared to the TLR4 WT HTV mice. Two-way ANOVA indicated that only ventilation was significant (P < 0.05), but analyzing TLR4 WT HTV and TLR4 KO HTV with Student’s t-test indicated a statistical significance (P < 0.05). (c) The release of DNA in the BALF was markedly decreased in the TLR4 KO mice compared with the TLR4 WT mice after 4 h of ventilation. Two-way ANOVA indicated both TLR4 genotype and ventilation were significant, but there was no interaction ( < 0.05). < 0.05.

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