Plant Proteinase Inhibitor BbCI Modulates Lung Inflammatory Responses and Mechanic and Remodeling Alterations Induced by Elastase in Mice

Figure 1

rBbCI purification from cells lysate. (a) Molecular exclusion chromatography on a Superdex G-75 10/30 column equilibrated with 50 M Tris-HCl buffer containing 150 M NaCl, pH 8.0. (b) Reverse phase chromatograph, HPLC system -BondapakC18. SDS-PAGE (15%) from reverse phase chromatography on C18. (M) Molecular mass standards; (1) rBbCI (30 μg) treated with β-mercaptoethanol.

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