Upregulation of Mitf by Phenolic Compounds-Rich Cymbopogon schoenanthus Treatment Promotes Melanogenesis in B16 Melanoma Cells and Human Epidermal Melanocytes

Figure 1

Effect of Cymbopogon schoenanthus ethanol extract on melanogenesis in B16 cells. (a) Melanin content (bar graph) and the cell viability (line graph) of B16 cells treated without (control) or with C. schoenanthus ethanol extract (CYM) at various concentrations (1/10000, 1/1000, or 1/100 v/v). (b) The effect of CYM on the expression of the tyrosinase (TYR), tyrosinase-related protein 1 (TRP1), and dopachrome tautomerase (DCT). The signals were visualized using LI-COR Odyssey Infrared Imaging System after reaction with goat anti-mouse IRDye 680LT or goat anti-rabbit IRDye 800CW (LI-COR). Results represent the mean ± SD of triplicate samples. significant () difference between treated cells and control. (c) Protein quantification based on the band intensities from the western blot in (b) determined using the LI-COR system.

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