Upregulation of Mitf by Phenolic Compounds-Rich Cymbopogon schoenanthus Treatment Promotes Melanogenesis in B16 Melanoma Cells and Human Epidermal Melanocytes

Figure 2

Effect of Cymbopogon schoenanthus ethanol extract on melanogenesis in human epidermal melanocytes (HEM). (a) Cell proliferation evaluated using MTT Assay: HEM (3 × 10³ cells/well) were treated with C. schoenanthus ethanol extract (CYM) at various concentrations (0, 1/10000, 1/1000, or 1/100 v/v) and incubated for 48 h. (b) Melanin content was determined by seeding HEM in 100 mm dish (5 × 10⁵ cells/dish) and treated without (control) or with 1/1000 (v/v) CYM and incubated for 72 h. The bar graph indicates the melanin content (left-hand -axis) while the line graph indicates cell viability (right-hand -axis). (c) Expression of the tyrosinase (TYR), tyrosinase-related protein 1 (TRP1), and dopachrome tautomerase (DCT) was determined using western blotting. Total protein was extracted from HEM cultured in 100 mm Petri dish (3 × 10⁶ cells/dish) and treated without (Con) or with 400 nm alpha-melanocyte-stimulating hormone (α-MSH) or 1/1000 (v/v) CYM for 48 h. Results represent the mean ± SD of triplicate determinations. significant () difference between control and treated cells. (d) Protein quantification based on the band intensities from the western blot in (c) determined using the LI-COR system.

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