Identification of Sp1 as a Transcription Activator to Regulate Fibroblast Growth Factor 21 Gene Expression

Figure 1

Deletion analysis of FGF21 promoter in HepG2 and 3T3-L1 cells. Schematic diagram of reporter constructs containing the indicated promoter fragments of the mouse FGF21 gene is shown on (a). The numbering is relative to the transcription start site. The corresponding luciferase reporter assay results are shown at (b). The relative promoter activity is expressed as the ratio of each construct to the promoterless control pGL3-Basic. Data represent the mean ± SD of triplicate assays in three independent experiments. versus pGL3-Basic, versus p(−63 to +70)Luc, p(−99 to +70)Luc, p(−472 to +70)Luc, p(−906 to +70)Luc, and p(−1432 to +70)Luc.