Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2

Table 1

RPA primers and probes designed in this research.

Name	Sequence (5′ –3′)	Genome location (DQ231511)
PCV2 RPA Fe1	ATACCATAACCCAGCCCTTCTCCTACCACTCCCGC	443–477
PCV2 RPA Fe2	ATAACCCAGCCCTTCTCCTACCACTCCCGCTACTT	448–482
PCV2 RPA Fe3	AAACCTGTCCTAGATTCCACTATTGATTACTTCCA	490–524
PCV2 RPA Re1	TTGTATTCCTGGTCGTATATACTGTTTTCGAACGC	601–635
PCV2 RPA Re2	ATATTGTATTCCTGGTCGTATATACTGTTTTCGAA	604–638
PCV2 RPA Re3	TTACACGGATATTGTATTCCTGGTCGTATATACTG	612–646
PCV2 RPA Pe	ATTACTTCCAACCAAACAACAAAAGAAATCAGCTG	515–569
	(FAM-dT)G(THF)C(BHQ1-dT)GAGACTACAAACTGC-C3 space
PCV2 RPA Fn1	ATACCATAACCCAGCCCTTCTCCTACCACTCCCGC	443–477
PCV2 RPA Fn2	ATAACCCAGCCCTTCTCCTACCACTCCCGCTACTT	448–482
PCV2 RPA Fn3	AAACCTGTCCTAGATTCCACTATTGATTACTTCCA	490–524
PCV2 RPA Rn1	biotin-TTGTATTCCTGGTCGTATATACTGTTTTCGAACGC	601–635
PCV2 RPA Rn2	biotin-ATATTGTATTCCTGGTCGTATATACTGTTTTCGAA	604–638
PCV2 RPA Rn3	biotin-TTACACGGATATTGTATTCCTGGTCGTATATACTG	612–646
PCV2 RPA Pn	FAM-TACTTCCAACCAAACAACAAAAGAAATCAGCTGTG	517–567
	-THF-CTGAGACTACAAACT-C3 space