Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2

Table 4

The performance of innuPREP MP basic kit on PCV2 positive samples was tested by real-time RPA assay, RPA LFD assay, and real-time qPCR assay, respectively.

Sample name	Real-time qPCR (CT)	Real-time RPA (min)	RPA LFD
Spleen 1	25	7	+
Spleen 2	27	7	+
Spleen 3	29	7.3	+
Spleen 4	30	7.6	+
Inguinal lymph node 1	28	7	+
Inguinal lymph node 2	32	8.3	+
Inguinal lymph node 3	31	8	+
Inguinal lymph node 4	29	7.6	+
Inguinal lymph node 5	26	6.3	+
Inguinal lymph node 6	26	6.6	+
Tonsil 1	30	8	+
Tonsil 2	31	8.3	+
Tonsil 3	25	6.3	+
Tonsil 4	25	6.6	+
Tonsil 5	26	6.6	+
Tonsil 6	27	7.3	+
Tonsil 7	27	7.6	+
Tonsil 8	28	7.3	+
Tonsil 9	24	6	+
Lung 1	24	6.3	+
Lung 2	24	6.6	+
Lung 3	29	7	+
Lung 4	22	6.3	+
Lung 5	22	6.6	+
Lung 6	30	8.3	+
Lung 7	30	8.6	+
Serum 1	32	9	+
Serum 2	32	9.3	+
Serum 3	27	7.6	+
Serum 4	29	7.6	+
Serum 5	25	7.3	+