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BioMed Research International
Volume 2017, Article ID 8415907, 10 pages
Research Article

The Prevalence and Replication Capacity of a Tibetan Dominant HBV Strain, C/D Recombinant

1Center of Infectious Diseases, West China Hospital of Sichuan University, Chengdu 610041, China
2Department of Science and Technology, West China Hospital of Sichuan University, Chengdu 610041, China

Correspondence should be addressed to Hong Tang; moc.liamtoh@8916gnath

Received 20 March 2017; Revised 16 May 2017; Accepted 24 May 2017; Published 21 June 2017

Academic Editor: Jorge F. Quarleri

Copyright © 2017 Lingyao Du et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study aimed to estimate the distribution of hepatitis B virus (HBV) C/D recombinant in Han and Tibet patients with chronic hepatitis B (CHB) and then learn such strain’s replication capacity in vivo. A total of 331 serum samples were collected from Han outpatients from Sichuan Province and Tibetan outpatients from Tibet. Viral genotypes in these samples were identified. An HBV replicative plasmid of C/D recombinant was constructed with selected genome. Sequentially, HBV replicative mouse models were established and the replication capacity of the viral strain was studied in vivo. In the 314 Han patients, 66% (207) were infected by genotype B strain while 31% (96) were by genotype C strain. Only 1% (3) were by C/D recombinant. In the 17 Tibetan patients, 41% (7) were by genotype D and 35% (6) by C/D recombinant. A plasmid with 1.3 copies of C/D recombinant genome was constructed. And its replication intermediates were found at similar levels to that of genotype D strain. Thus, C/D recombinant, the dominant viral strain in Tibet, was rather rare in the genotype B predominated Han patients from Sichuan Province. And the C/D recombinant replicated at a similar level to viral strain of genotype D in vivo.