The Prevalence and Replication Capacity of a Tibetan Dominant HBV Strain, C/D Recombinant

Table 3

Primers for construction of pHBV4.1C/D with viral genome in serum sample.

Primers for amplification of HBV genome
Upstream	5′-CCGGAAAGCTTGAGCTCTTCTTTTTCACCTCTGCCTAATCA-3′
Downstream	5′-CCGGAAAGCTTGAGCTCTTCAAAAAGTTGCATGGTGCTGG-3′
Primers for amplification of 2.4 Kb fragment
Upstream	5′-CCTGCTTTAATGCCTTTGTATGC-3′
Downstream	5′-TCCACCACGAGTCTAGACTCTGTGG-3′
Primers for amplification of 1.7 Kb fragment
Upstream	5′-CCACAGAGTCTAGACTCGTGGTGGA-3′
Downstream	5′-CGGTGTCGAGGAGATCTCGAATAGA-3′