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BioMed Research International
Volume 2017 (2017), Article ID 8428926, 9 pages
Research Article

Expressions Profiles of the Proteins Associated with Carbohydrate Metabolism in Rat Liver Regeneration

Li Yin,1,2,3 Cuifang Chang,1,2 and Cunshuan Xu1,2

1College of Life Science, Henan Normal University, Xinxiang, Henan Province 453007, China
2State Key Laboratory Cultivation Base for Cell Differentiation Regulation and Henan Bioengineering Key Laboratory, Henan Normal University, Xinxiang, Henan Province 453007, China
3Luohe Medical College, Luohe, Henan Province 462002, China

Correspondence should be addressed to Cunshuan Xu

Received 18 February 2017; Revised 11 May 2017; Accepted 28 May 2017; Published 2 July 2017

Academic Editor: Yu-Chang Tyan

Copyright © 2017 Li Yin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Liver has a very amazing ability to regenerate from the remnant liver after injury or partial hepatectomy (PH). Carbohydrate metabolism plays a critical role in regeneration. Many signaling pathways are involved in the metabolism process. We analyzed the changes of proteins at 0–36 h after PH in rats using isobaric tags for relative and absolute quantitation (iTRAQ) coupled with LC-MS/MS-based quantitative proteomics strategy. The results showed that 110 proteins and 5 signaling pathways related to carbohydrate metabolism in rat LR changed significantly. Based on a motif discovery method performed by iRegulon, we identified for the first time that the transcription factor SPIB whose motif was enriched among the differentiated genes associated with carbohydrate metabolism may play an important role in liver regeneration for the first time. The findings of this research provide a molecular basis for further unrevealing the mechanism of regeneration at priming stage (0–6 h) and proliferation stage (6–36 h) of LR in rats. At the same time, our studies provide more novel evidence for the signaling pathways which regulate carbohydrate metabolism from proteomics level. This study can provide some new thinking of liver regeneration and treatment of diseases associated with glucose metabolism.