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BioMed Research International
Volume 2017, Article ID 8459287, 10 pages
https://doi.org/10.1155/2017/8459287
Research Article

Paeoniflorin, the Main Active Ingredient of Shuyu Capsule, Inhibits Cav1.2 and Regulates Calmodulin/Calmodulin-Dependent Protein Kinase II Signalling

1Experiment Center, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
2College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
3School of Preclinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
4Fengtai Maternal and Children’s Health Hospital of Beijing, Beijing 100069, China

Correspondence should be addressed to Chunhong Song; moc.621@25546-hcs and Mingqi Qiao; moc.361@iqgnimq

Received 26 August 2017; Accepted 12 November 2017; Published 6 December 2017

Academic Editor: Yiannis Kourkoutas

Copyright © 2017 Chunhong Song et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to explore the mechanism underlying the antidepression activity of paeoniflorin, the main active ingredient of paeony extract and Shuyu capsules, and determine its effect on the calmodulin/calmodulin-dependent protein kinase II (CaM/CaMKII) signalling pathway and on the possible target, the voltage-gated calcium channel (). Rats at the nonacceptance stage were selected for premenstrual syndrome (PMS) depression modelling. Behavioural assays were used for model testing. Rats were given Shuyu capsules, paeony extract, and bupleurum. Western blot analysis was used to assess the expression levels of calcium voltage-gated channel subunit alpha 1 C (CACNA1C), brain-derived neurotrophic factor, and CaM/CaMKII signalling pathway proteins. Intracellular Ca2+ concentration in CHO cell line was measured using Fluo-4-AM and whole-cell patch clamps. The PMS depression model was successfully established and demonstrated that Shuyu can mitigate depressive behaviour in a rat PMS model. Paeony extract did not affect CACNA1C protein expression in rat hippocampi but did affect 1.2-mediated CaM/CaMKII signalling pathways. Paeoniflorin significantly inhibited KCl-induced increases in intracellular Ca2+ concentration and 1.2 current density. Further, it may function via the CaM/CaMKII pathway and its downstream signalling molecules by regulating 1.2, thus playing an important role in the treatment and alleviation of affective disorders.