Dexamethasone Modulates Nonvisual Opsins, Glucocorticoid Receptor, and Clock Genes in Danio rerio ZEM-2S Cells

Figure 8

Time course of per1b and cry1b expression in D. rerio ZEM-2S cells exposed to LD (12 : 12) cycles. (a) per1b and (b) cry1b in nonmanipulated cells; (c) per1b in cells subject to 6 medium changes (blue bars) or 3 DEX pulses (red bars); (d) cry1b in cells subject to 6 medium changes (blue bars) or 3 DEX pulses (red bars). Each value is the mean ( flasks), ±SEM, of transcripts normalized by ribosomal 18S RNA. (a) and (b) are expressed relative to 24 h average. (c) and (d) relative to 24 h average of 6 medium changes. Temporal variation: A is different from B (, one way ANOVA, followed by Tukey posttest), and DEX-treated group is different from the respective control (, two way ANOVA, followed by Bonferroni posttest).

(a) per1b

(b) cry1b

(c) per1b

(d) cry1b