Dexamethasone Modulates Nonvisual Opsins, Glucocorticoid Receptor, and Clock Genes in Danio rerio ZEM-2S Cells

Figure 9

Time course of per1b and cry1b expression in D. rerio ZEM-2S cells subject to DD. (a) per1b and (b) cry1b in nonmanipulated cells; (c) per1b in cells subject to 10 medium changes (blue symbols) or 5 DEX pulses (red symbols); (d) cry1b in cells subject to 10 medium changes (blue symbols) or 5 DEX pulses (red symbols). Each value is the mean ( flasks), ±SEM, of transcripts normalized by ribosomal 18S RNA. (a) and (b) are expressed relative to 24 h average, (c) and (d) relative to 24 h average of 6 medium changes. A significantly different from B demonstrated by one way ANOVA, followed by Tukey (). Asterisk means significantly different from the respective control at the same time point (, two way ANOVA followed by Bonferroni).

(a) per1b

(b) cry1b

(c) per1b

(d) cry1b