Dexamethasone Modulates Nonvisual Opsins, Glucocorticoid Receptor, and Clock Genes in Danio rerio ZEM-2S Cells

Table 1

Primers and probes for qPCR assays.

Templates; (access number)	Primers and probes	Final concentration
18S RNA; X03205.1	Forward: 5′-CGGCTACCACATCCAAGGAA-3′	50 nM
	Reverse: 5′-GCTGGAATTACCGCGGCT-3′	50 nM
	Probe: 5′TexRd/TGCTGGCACCAGACTTGCCCTC/BHQ_2/-3′	50 nM
opn4m1; GQ925715.1	Forward: 5′-GGGCAACTTCCTGGTCATCTATG-3′	300 nM
	Reverse: 5′- AGGGAACTGACACTGGAACCA-3′	300 nM
	Probe: 5′-/5Cy5/AGGAGCCGGACCCTGAGGACCC/BHQ_2/-3′	200 nM
opn4m2; AY078161	Forward: 5′-GCGATTGTCTTCTGCCTCTGA-3′	300 nM
	Reverse: 5′-AGGGAACTGACACTGGAACCA-3′	300 nM
	Probe: 5′-/6FAM/AGTGATTCTTGCTGGACTGAGAGTGAGGCT/BHQ_1/-3′	200 nM
gr; NM_001020711.2	Forward: 5′-GAGGAGAACTCCAGCCAGAAC-3′	300 nM
	Reverse: 5′-TTCACAAAGGTGTAGAAGCAGAAG-3′	300 nM
	Probe: 5′-/5HEX/AGGAGTCCACCCACCAAGTCGTGC/BHQ_1/-3′	200 nM
per1b; NM_212439.2	Forward: 5′-AGCTCAAACTCTCACAGCCCTT-3′	300 nM
	Reverse: 5′-TCAGAGCTGGCACTCAACAGA-3′	300 nM
	Probe: 5′-/5Cy5/TCCACCCAGCAGTTCTCTGGCATACA/BHQ_2/-3′	200 nM
cry1b; NM_131790.4	Forward: 5′-CGTCTCTGGAGGAGCTCGG-3′	300 nM
	Reverse: 5′-TCTCCCCCGGGCCAC-3′	300 nM
	Probe: 5′-/5HEX/TTTGAAACAGAGGGACTGTCCACTGCTG/BHQ_1/ 3′	200 nM

FAM = carboxyfluorescein; TexRd = Texas Red fluorophore; Cy5 = Cyanine 5 fluorophore; Hex = 6-carboxy-2,4,4,4,7,7-hexachlorofluorescein succinimidyl ester fluorophore; BHQ_1 = black hole quencher 1; BHQ_2 = black hole quencher 2.
Gene nomenclature according to http://www.genenames.org for Homo sapiens; http://www.informatics.jax.org/genes.shtml for Mus musculus; https://wiki.zfin.org for Danio rerio.