Effects of Bacillus Serine Proteases on the Bacterial Biofilms

Figure 1

S. marcescens SR 41-8000 biofilm formation on catheters incubated in different growth media. ((a)–(c)) Catheters were incubated at 30°C for 6 days, fixed with 2.5% glutaraldehyde, and photographed at 5,000x magnification. Bars: 1 μm. (a) Biofilms grown in LB broth. (b) Biofilms grown in MBD/0.5% glucose medium. (c) Biofilms grown in MH broth. (d) Biofilm formation in LB broth, MBD/0.5% glucose medium, and MH broth evaluated in crystal violet assay. (e) Morphology of free-living S. marcescens cells. Sample preparation of planktonic cells was performed as described in Section 2. Scanning electron microscopy image of the free-living S. marcescens cells was taken at 10,000x magnification. Bars: 1 μm. Experiments were performed in triplicate and repeated three times. Error bars represent standard deviations.

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