Effects of Bacillus Serine Proteases on the Bacterial Biofilms

Figure 2

Morphological changes of S. marcescens cells during biofilm formation. Initial steps of the biofilm formation result in the appearance of elongated cells on the catheter surface after 3 days of incubation in MH broth at 30°C. Images were taken at 10,000x (a) and 20,000x (b) magnification. Bars are 1 μm and 200 nm, respectively. Extracellular matrix production by S. marcescens cells analyzed after 3 (c), 4 (d), and 7 (e) days of incubation progresses over the time. Images were taken at 10,000x (c, e) and 20,000x (d) magnification. Bars are 1 μm and 200 nm, respectively.

(a)

(b)

(c)

(d)

(e)