Effects of Bacillus Serine Proteases on the Bacterial Biofilms

Figure 4

Time-course of the biofilm formation by S. marcescens SR 41-8000. Biofilm formation on catheters’ surface was evaluated by SEM ((a)–(d)) after 1 (a), 3 (b), 5 (c), and 7 (d) days of cultivation in MH broth. Images were taken at 5,000x magnification. Bars: 1 μm. Quantitative analysis of biofilm formation in crystal violet assay (e) was performed over 7 days of growth as described in Section 2. Experiments were performed in triplicate and repeated twice. Error bars represent standard deviations.

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