Effect of Gamma Rays on Sophora davidii and Detection of DNA Polymorphism through ISSR Marker

Table 2

List of primers, number of amplified products, polymorphic bands, and polymorphism percentage.

Number	Primers	Sequence (5′–3′)	TAP	NPB	PPB (%)
1	UBC857	AGAGAGAGAGAGAGAGYG	17	12	70.59
2	UBC818	CACACACACACACACAG	7	2	28.57
3	UBC850	GTGTGTGTGTGTGTGTYC	9	4	44.44
4	UBC840	GAGAGAGAGAGAGAGAYT	10	2	20.00
5	UBC812	GAGAGAGAGAGAGAGAA	7	1	14.29
6	ISSR04	GAGAGAGAGAGAGAGACT	10	2	20.00
7	ISSR11	ACACACACACACACACTG	11	8	72.73
8	ISSR21	GAGAGAGAGAGAGAGATG	7	2	28.57
9	ISSR25	ACACACACACACACACCT	10	4	40.00
10	ISSR24	ACACACACACACACACCA	13	7	53.85
11	ISSR36	GCGTCTCTCTCTCTCTC	8	2	25.00
12	ISSR47	AGAAGAAGAAGAAGAAGAAGA	7	4	57.14
13	ISSR02	AGAGAGAGAGAGAGAGCC	12	10	83.33
14	ISSR05	GAGAGAGAGAGAGAGACC	7	5	71.43
15	ISSR06	GAGAGAGAGAGAGAGACG	12	9	75.00
16	ISSR08	GTGTGTGTGTGTGTGTCC	15	14	93.33
17	ISSR12	CCCTCCCTCCCTCCCT	3	1	33.33
18	ISSR14	CTTCACTTCACTTCA	7	3	42.86
19	ISSR10	ACACACACACACACACTA	4	1	25.00
20	ISSR15	GGAGAGGAGAGGAGA	7	1	14.29
		Total	183	94	913.75
		Mean	9.15	4.7	45.69

TAP, total amplified products; NPB, number of polymorphic bands; PPB, percentage of polymorphic bands.