An ECM-Mimicking, Mesenchymal Stem Cell-Embedded Hybrid Scaffold for Bone Regeneration

Figure 5

Flow cytometry analysis of MSCs cultures. Adipose-derived MSCs were cultured for 28 days in basic medium, trypsinized, and stained with fluorescent antibodies to mesenchyme-specific markers (CD73, CD105, and CD90; framed), hematopoietic markers (CD34, CD45), and leukocyte markers (CD11b, CD19, and HLA-DR) and analyzed using flow cytometer to confirm their MSC phenotype.