Research Article
Osteogenic Differentiation Capacity of In Vitro Cultured Human Skeletal Muscle for Expedited Bone Tissue Engineering
Table 1
List and sequences of primers used for analysis of mRNA expression. All sequences are for human genes. F, forward; R, reverse.
| Gene | Primer sequences (5′-3′) | Product sizes (bp) | Accession number | Primer efficiency (%) |
| 18 S rRNA | F: CGGCTACCACATCCAAGGAA | 187 | NR 003286.2 | 98 | R: GCTGGAATTACCGCGGCT | ALP | F: CGTGGCTAAGAATGTCATCATGTT | 90 | NM 000478.3 | 97 | R: TGGTGGAGCTGACCCTTGA | Runx2 | F: GCCTTCAAGGTGGTAGCCC | 67 | NM 001024630.2 | 96 | R: CGTTACCCGCCATGACAGTA | COL1A1 | F: TGGTTTCGACTTCAGCTTCC | 92 | NM 000088.3 | 95 | R: GAACCACATTGGCATCATCA | OCN | F: GTAGTGAAGAGACCCAGGCG | 99 | NM 199173.2 | 97 | R: ATTGAGCTCACACACCTCCC | BSP | F: GGCCTGTGCTTTCTCAATGAA | 83 | NM 004967.3 | 87 | R: GCCTGTACTTAAAGACCCCATTTTC |
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ALP, alkaline phosphatase; Runx2, runt-related transcription factor 2; COL1A1, collagen type I, alpha 1 chain; OCN, osteocalcin; BSP, bone sialoprotein.
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