Osteogenic Differentiation Capacity of In Vitro Cultured Human Skeletal Muscle for Expedited Bone Tissue Engineering

Table 1

List and sequences of primers used for analysis of mRNA expression. All sequences are for human genes. F, forward; R, reverse.

Gene	Primer sequences (5′-3′)	Product sizes (bp)	Accession number	Primer efficiency (%)
18 S rRNA	F: CGGCTACCACATCCAAGGAA	187	NR 003286.2	98
	R: GCTGGAATTACCGCGGCT
ALP	F: CGTGGCTAAGAATGTCATCATGTT	90	NM 000478.3	97
	R: TGGTGGAGCTGACCCTTGA
Runx2	F: GCCTTCAAGGTGGTAGCCC	67	NM 001024630.2	96
	R: CGTTACCCGCCATGACAGTA
COL1A1	F: TGGTTTCGACTTCAGCTTCC	92	NM 000088.3	95
	R: GAACCACATTGGCATCATCA
OCN	F: GTAGTGAAGAGACCCAGGCG	99	NM 199173.2	97
	R: ATTGAGCTCACACACCTCCC
BSP	F: GGCCTGTGCTTTCTCAATGAA	83	NM 004967.3	87
	R: GCCTGTACTTAAAGACCCCATTTTC

ALP, alkaline phosphatase; Runx2, runt-related transcription factor 2; COL1A1, collagen type I, alpha 1 chain; OCN, osteocalcin; BSP, bone sialoprotein.