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BioMed Research International
Volume 2017 (2017), Article ID 8720367, 10 pages
Research Article

Effects of Liver Fibrosis Progression on Tissue Relaxation Times in Different Mouse Models Assessed by Ultrahigh Field Magnetic Resonance Imaging

1Clinic for Diagnostic and Interventional Radiology, Saarland University Medical Center, Kirrberger Str. 100, Bdg. 50.1, 66421 Homburg, Germany
2Department of Medicine, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA
3Department of Internal Medicine II, Saarland University, Saarland University Medical Center, Bdg. 77, Kirrberger Str. 100, 66421 Homburg, Germany
4Institute of Pathology, University Hospital Bonn, Bdg. 62, Sigmund-Freud Str. 25, 53127 Bonn, Germany

Correspondence should be addressed to Peter Fries

Received 11 October 2016; Accepted 14 December 2016; Published 18 January 2017

Academic Editor: Kim Bridle

Copyright © 2017 Andreas Müller et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Recently, clinical studies demonstrated that magnetic resonance relaxometry with determination of relaxation times T1 and may aid in staging and management of liver fibrosis in patients suffering from viral hepatitis and steatohepatitis. In the present study we investigated T1 and in different models of liver fibrosis to compare alternate pathophysiologies in their effects on relaxation times and to further develop noninvasive quantification methods of liver fibrosis. MRI was performed with a fast spin echo sequence for measurement of T1 and a multigradient echo sequence for determination of . Toxic liver fibrosis was induced by injections of carbon tetrachloride (1.4 mL CCl4 per kg bodyweight and week, for 3 or 6 weeks) in BALB/cJ mice. Chronic sclerosing cholangitis was mimicked using the ATP-binding cassette transporter B4 knockout (Abcb4) mouse model. Untreated BALB/cJ mice served as controls. To assess hepatic fibrosis, we ascertained collagen contents and fibrosis scores after Sirius red staining. T1 and correlate differently to disease severity and etiology of liver fibrosis. shows significant decrease correlating with fibrosis in CCl4 treated animals, while demonstrating significant increase with disease severity in Abcb4 mice. Measurements of T1 and may therefore facilitate discrimination between different stages and causes of liver fibrosis.