Evaluation of Decalcification Techniques for Rat Femurs Using HE and Immunohistochemical Staining

Figure 1

Representative microstructure ((a); ×100) and histological images (b) of HE staining showed the influences of different decalcified solutions on morphological structure preservation of rat femurs. 1 to 3 represent superficial, cartilage, and subchondral zone, respectively. HFA represents hydrochloric acid/formic acid.

(a)

(b)