Evaluation of Decalcification Techniques for Rat Femurs Using HE and Immunohistochemical Staining

Table 1

The ingredients and preparation of different decalcifying agents.

	Decalcifying solution 1	Decalcifying solution 2	Decalcifying solution 3	Decalcifying solution 4
Decalcifying agents	10% EDTA	3% nitric acid	5% nitric acid	8% hydrochloric acid/formic acid
Preparation	100 g EDTA and 10 g sodium hydroxide	3 mL nitric acid	5 mL nitric acid	8 mL hydrochloric acid and 8 mL formic acid
Distilled water	Add to 1000 mL	Add to 100 mL	Add to 100 mL	Add to 100 mL
pH	7.4	—	—	—