Exploring the Association of Surface Plasmon Resonance with Recombinant MHC:Ig Hybrid Protein as a Tool for Detecting T Lymphocytes in Mice Infected with Leishmania (Leishmania) amazonensis

Figure 2

Profiles of lesion growth and CD4⁺/CD8⁺ T lymphocyte populations in BALB/c mice infected with L. (L.) amazonensis. Animals were inoculated subcutaneously with 1.0 × 10⁵ promastigotes in the plantar cushion of the left hind paw and lesions were monitored weekly by measuring the height () and width () of the cushion with a pachymeter (a). The results represent the lesion size ( × ) mean ± standard deviation (SD) of five animals, in millimeters (mm). The immunological profile of the popliteal lymph node cells was determined by flow cytometry in groups of infected (b) and noninfected (c) animals. The values of the CD8⁺/CD4⁺ ratio indicate an increase in the CD8+ lymphocyte population in animals infected with L. (L) amazonensis. The data are representative of three independents experiments performed in triplicate.

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