Exploring the Association of Surface Plasmon Resonance with Recombinant MHC:Ig Hybrid Protein as a Tool for Detecting T Lymphocytes in Mice Infected with Leishmania (Leishmania) amazonensis

Figure 3

Graphic representations of flow cytometry. Dot plots were based on the evaluation of the gate analysis (a and b), delimiting the area of lymphocytes; we may infer that the morphology of the cells remained preserved throughout the study, which indicates that there were little cell death and suffering during the procedure to obtain them. The data are for the noninfected (a) and infected (b) mice group with L. (L.) amazonensis. Histogram superposition (c and d) shows the control isotype in the low fluorescence intensity, corresponding to negative region (0 to 10¹), as signals above this range were considered positive reactions (10²–10³). This distance between areas of negative and positive signal is indicative of a good reaction receptor blocking unspecific binding. Moreover, the data show that there is a difference in the percentage of lymphocytes when comparing the noninfected (c) and infected (d) mice group with L. (L.) amazonensis. These data are representative of three replicates of lymph node cells analysis from 4th week of mice infection.

(a)

(b)

(c)

(d)