Exploring the Association of Surface Plasmon Resonance with Recombinant MHC:Ig Hybrid Protein as a Tool for Detecting T Lymphocytes in Mice Infected with Leishmania (Leishmania) amazonensis

Figure 5

Interaction between immobilized DimerX/peptides complexes and lymph node cells preparations. The assays were performed with cells from L. (L.) amazonensis-infected (a) or noninfected (b) mice, which were streamed over a sensor chip containing the immobilized complexes. After each step of cell binding, the cells and peptides were removed with 30 mM HCl solution, leaving α1/α2 domains of the DimerX proteins free for a further interaction cycle. Sensorgram graphs are representative of all regeneration cycles () and show the RU values for assayed peptides ( dark blue; dark red; green; purple; blue; orange) during 151 seconds. The data are representative of three independents experiments performed in triplicate.

(a)

(b)