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BioMed Research International
Volume 2017, Article ID 9153403, 13 pages
https://doi.org/10.1155/2017/9153403
Research Article

Anticancer Effect of a Novel Octahydropyrazino[2,1-a:5,4-a′]diisoquinoline Derivative and Its Synergistic Action with Nigella sativa in Human Gastric Cancer Cells

1Department of Biotechnology, Medical University of Białystok, Kilinskiego 1, 15-089 Białystok, Poland
2Department of Synthesis and Technology of Drugs, Medical University of Białystok, Kilinskiego 1, 15-089 Białystok, Poland
3Herbarium of Department of Pharmacognosy, Medical University of Białystok, Kilinskiego 1, 15-089 Białystok, Poland

Correspondence should be addressed to Agnieszka Gornowicz; lp.ude.bmu@zciwonrog.akzseinga

Received 4 August 2017; Revised 9 November 2017; Accepted 27 November 2017; Published 26 December 2017

Academic Editor: Anne Hamburger

Copyright © 2017 Anna Czajkowska et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Many studies have shown that naturally occurring compounds may support prevention and treatment of various diseases, including cancer. Pharmacological investigations revealed a wide spectrum of Nigella sativa biological activities. Combining natural compounds together with synthetic drugs may increase the anticancer activity and limit severe side effects of such a treatment and may be an alternative to monotherapy. The aim of the study was to evaluate the cytotoxic and proapoptotic effects of a novel octahydropyrazino[2,1-a:5,4-a′]diisoquinoline derivative and its effect in combination with Nigella sativa seed oil or extract in human gastric cancer cells (AGS). Etoposide was used as a reference. Our studies proved that combination strategy based on novel octahydropyrazino[2,1-a:5,4-a′]diisoquinoline derivative (OM-90) with Nigella sativa seed oil or extract represents the strongest efficacy in AGS cancer cells as compared to monotherapy and combined treatment with Nigella sativa seed oil or extract together with etoposide. Such a combination also leads to the activation of mitochondrial pathway, which plays a significant role in molecular mechanism of induction of apoptosis by these compounds.