Anticancer Effect of a Novel Octahydropyrazino[2,1-a:5,4-a′]diisoquinoline Derivative and Its Synergistic Action with Nigella sativa in Human Gastric Cancer Cells

Figure 10

Proapoptotic properties of Nigella sativa seed oil (2.4 mg/mL), Nigella sativa seed extract (0.5 mg/mL), OM-90 (20 μM), etoposide (20 μM), Nigella sativa seed oil + OM-90 (2.4 mg/mL + 20 μM), Nigella sativa seed oil + etoposide (2.4 mg/mL + 20 μM), Nigella sativa seed methanolic extract + OM-90 (0.5 mg/mL + 20 μM), and Nigella sativa seed methanolic extract + etoposide (0.5 mg/mL + 20 μM) in AGS gastric adenocarcinoma cells after 24 hours of incubation, obtained using dual acridine orange/ethidium bromide (AO/EB) fluorescent staining. Images were taken using a microscope with an inverted camera (Nikon Eclipse Ti, Nikon Instruments Inc., Melville, NY, USA) at 100x magnification.