Natural Scaffolds for Regenerative Medicine: Direct Determination of Detergents Entrapped in Decellularized Heart Valves

Figure 1

Overlay of chromatograms obtained by running 6 mL of the defatted aortic leaflet digest before (black line) and after (blue line) addition of 0.3 mg deoxycholate (DOC). The two chromatograms were obtained under the following conditions: flow rate, 1 mL/min; eluent A, 0.05% TFA in H₂O MilliQ; eluent B, 0.05% TFA in CH₃CN; gradient, from 0% to 80% of eluent B in 40 min; λ, 200 nm. AU = optical density.