Research Article

Natural Scaffolds for Regenerative Medicine: Direct Determination of Detergents Entrapped in Decellularized Heart Valves

Table 3

Concentration (% w/lyophilized leaflet weight) of residual detergent in decellularized aortic and pulmonary leaflets; single deoxycholate (S-DOC), combined deoxycholate/SDS (C-DOC/SDS), triton/cholate (TriCOL), and triton/taurodeoxycholate (Tri-TDOC).

Decellularization procedureAnalyteAortic leaflets 
mg/100 mg
Pulmonary leaflets
mg/100 mg
Aortic versus pulmonary
value

S-DOCDOC1.13 ± 0.050.32 ± 0.005 < 0.0001
C-DOC/SDSDOC0.92 ± 0.120.31 ± 0.02 < 0.001
TriCOLCOL0.39 ± 0.030.13 ± 0.07 < 0.005
Tri-TDOCTDOC0.09 ± 0.0020.06 ± 0.005 < 0.001