Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics

Table 3

Summary of the advantages and disadvantages of solid-phase extraction methods.

Material	Molecule of affinity	Advantage	Disadvantage	Reference
(1) Silica matrices	DNA, RNA	High-purity DNA, easy to perform, and reproducible	Unable to recover small DNA fragments; one-time use	[52]
(2) Glass particles	DNA, protein	Simple, sensitive, and reproducible	High cost; requirement of equipment	[1, 52]
(3) Diatomaceous earth	DNA, RNA	Reduced pipetting error, shorter protocol (less time and steps)	High cost	[53]
(4) Magnetic beads	DNA, RNA	No centrifugation, best choice for automation, virtually equipment-free	Interference in PCR amplification	[56]
(5) Anion exchange material	DNA, RNA	Reusable resins	Presence of high-salt concentrations	[58]
(6) Cellulose matrix	DNA, RNA	Easy to use and storage	Extraction protocols being complex and prone to error	[60]