Figure 4: Immunohistochemistry analysis of regenerated pulp tissue. (a–c) Immunohistochemistry analysis showed polygonal cells layer highly expressing DSPP, which is a marker of odontoblast-like cells; no odontoblast, and mineralized tissue formation could be found in the control group. (d) Quantitive analysis of DSPP positive cells in natural pulp, control, and recellularized matrix group. Values are mean ± SD, . One-way ANOVA was used to determine statistical significance, . Scale bars: 100 μm. D: dentin; P: pulp; Od: odontoblasts; scale bars: 50 μm.