Heterologous Secretory Expression and Characterization of Dimerized Bone Morphogenetic Protein 2 in Bacillus subtilis

Figure 6

Biological activity of rhBMP2 monomer and dimer estimated by ALP assay and measured at nmol/ml/min. (a) Standard curve of ALP. -axis showing the pNPP conc. (nmol) and -axis showing the absorbance at 405 nm. (b) Different concentrations of rhBMP2 monomer and dimer (0, 50, 100, 200, and 400 ng/ml) were tested and maximum activity was observed at 200 ng/ml, respectively, in dose dependent manner. Higher dose (400 ng/ml) showed reduce activity. Positive control (human BMP standard) was tested at 100 and 500 ng/ml doses and showed reduce activity at 500 ng/ml. Negative control (DMEM) showed negligible ALP activity.