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BioMed Research International
Volume 2017, Article ID 9390565, 10 pages
Research Article

Enhancement of Angiogenesis by Ultrasound-Targeted Microbubble Destruction Combined with Nuclear Localization Signaling Peptides in Canine Myocardial Infarction

1Department of Ultrasound Imaging, Renmin Hospital of Wuhan University, Wuhan 430000, China
2Department of Ultrasound Imaging, Affiliated Hospital of Jining Medical University, Jining 272000, China

Correspondence should be addressed to Qing Zhou; moc.liamtoh@ude.hw.uohzgniq

Received 23 April 2017; Revised 23 August 2017; Accepted 7 September 2017; Published 12 November 2017

Academic Editor: Magali Cucchiarini

Copyright © 2017 Jingjing Cui et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. This study aimed to develop a gene delivery system using ultrasound-targeted microbubbles destruction (UTMD) combined with nuclear localization signal (NLS) and investigate its efficacy and safety for therapeutic angiogenesis in canine myocardial infarction (MI) model. Methods. Fifty MI dogs were randomly divided into 5 groups and transfected with Ang-1 gene plasmid: (i) group A: only injection of microbubbles and Ang-1 plasmid; (ii) group B: only UTMD mediated gene transfection; (iii) group C: UTMD combined with classical NLS mediated gene transfection; (iv) group D: UTMD combined with mutational NLS mediated transfection; and (v) group E: UTMD combined with classical NLS in the presence of a nucleus transport blocker. The mRNA and protein expression of Ang-1 gene, microvessel density (MVD) cardiac troponin I (cTnI), and cardiac function were determined after transfection. Results. The expression of mRNA and protein of Ang-1 gene in group C was significantly higher than that of the other groups (all P < 0.01). The MVD of group C was 10.2-fold of group A and 8.1-fold of group E (P < 0.01). The cardiac function in group C was significant improvement without cTnI rising. Conclusions. The gene delivery system composed of UTMD and NLS is efficient and safe.