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BioMed Research International
Volume 2017 (2017), Article ID 9436280, 12 pages
https://doi.org/10.1155/2017/9436280
Research Article

The Anti-Inflammatory Activity of Toonaciliatin K against Adjuvant Arthritis

1Department of Traditional Chinese Medicine, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital, Fudan University, Shanghai 200040, China
2Department of Orthopaedics, Longhua Hospital, Shanghai Traditional Chinese Medicine University, Shanghai 200032, China
3Department of Emergency, Jiading Hospital of Traditional Chinese Medicine, Shanghai 201899, China
4Department of Traditional Chinese Medicine, Sixth People’s Hospital, Jinshan Branch, Shanghai 201500, China

Correspondence should be addressed to XinGang Lu

Received 28 April 2017; Revised 18 July 2017; Accepted 1 August 2017; Published 17 October 2017

Academic Editor: Adair Santos

Copyright © 2017 HaiXing Gou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Toonaciliatin K is a natural limonoid purified from the Toona ciliata Roem. var. ciliata (Meliaceae). This study is to reveal the inflammatory suppression effect of toonaciliatin K and further the intrinsic mechanism. Firstly, anti-inflammatory effect of toonaciliatin K was evaluated in lipopolysaccharide- (LPS-) induced RAW264.7 cells. RT-PCR results indicated that the mRNA expressions of TNF-α, IL-6, and IL-1β were downregulated by toonaciliatin K. The toonaciliatin K inhibited TNF-α, IL-6, and IL-1β levels stimulated by LPS. Furthermore, LPS elicited the excess iNOS and COX-2 mRNA and protein production and toonaciliatin K attenuated the excess production. Western blot assay demonstrated that MAPK and NF-κB signaling pathways play critical roles in the toonaciliatin K’s anti-inflammatory activity. Secondly, toonaciliatin K inhibited carrageenan-induced paw edema in rats. Thirdly, toonaciliatin K alleviated the paw swelling and improved arthritis clinical scores in the adjuvant arthritis rats. Toonaciliatin K decreased the proinflammatory cytokines levels and Mankin scores in adjuvant arthritis rats. The HE staining, safranin O-fast green, and toluidine blue staining results demonstrated that toonaciliatin K alleviated the histological changes of paw, for example, pannus formation, focal loss of cartilage, bone erosion, and presence of extra-articular inflammation. Hence, toonaciliatin K is a promising agent for treatment of arthritis.