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BioMed Research International
Volume 2017, Article ID 9474573, 11 pages
https://doi.org/10.1155/2017/9474573
Research Article

Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

1Department of Implantology, Stomatology Hospital, School of Medicine, Zhejiang University, Yan’an Road, Hangzhou, China
2Department of Oral Medicine, Stomatology Hospital, School of Medicine, Zhejiang University, Yan’an Road, Hangzhou, China
3Department of Oral and Maxillofacial Surgery, Stomatology Hospital, School of Medicine, Zhejiang University, Yan’an Road, Hangzhou, China

Correspondence should be addressed to Guoli Yang; moc.nuyila@7791il_oug

Received 29 July 2016; Revised 28 October 2016; Accepted 2 November 2016; Published 9 January 2017

Academic Editor: Rei Shibata

Copyright © 2017 Zhiwei Jiang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Rat bone marrow mesenchymal stem cell sheets (rBMSC sheets) are attractive for cell-based tissue engineering. However, methods of culturing rBMSC sheets are critically limited. In order to obtain intact rBMSC sheets, a light-induced cell sheet method was used in this study. TiO2 nanodot films were coated with (TL) or without (TN) laminin-521. We investigated the effects of laminin-521 on rBMSCs during cell sheet culturing. The fabricated rBMSC sheets were subsequently assessed to study cell sheet viability, reattachment ability, cell sheet thickness, collagen type I deposition, and multilineage potential. The results showed that laminin-521 could promote the formation of rBMSC sheets with good viability under hyperconfluent conditions. Cell sheet thickness increased from an initial 26.7 ± 1.5 μm (day 5) up to 47.7 ± 3.0 μm (day 10). Moreover, rBMSC sheets maintained their potential of osteogenic, adipogenic, and chondrogenic differentiation. This study provides a new strategy to obtain rBMSC sheets using light-induced cell sheet technology.