Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

Figure 1

Adhesion assay of rBMSCs on nanodot films coated with (TL) or without (TN) laminin-521. Attached rBMSCs on different concentration of laminin-521-coated samples after incubation for 0.5 h (a) and 2 h (b) tested by Alamar Blue. Concentration of coated laminin-521 measured by micro BCA assay (c). Ability of adhesion measured by cytoskeleton (green) and nuclei (blue) double-labeled method after culturing on TN and TL samples for 2 h and 4 h (d), (e). Scale bar: 50 μm. .

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