Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

Figure 2

Morphology of rBMSCs cultured on TN or TL samples after seeding for intended time observed by phase-contrast microscope (a). Proliferation assay of rBMSC sheets treated with UV illumination or trypsin tested by Alamar Blue (b). Thickness of fabricated cell sheets at day 5, day 7, and day 10 observed by laser confocal microscopy (c). Oxidative damage of DNA assessed using 8-OH-dG assay (d). Live-dead staining of rBMSC sheets after illumination of UV365 for 30 min (e). rBMSC sheets with the treatment of 30% ethanol were stained as negative control group (f). , . Scale bar: (a): 200 m; (e), (f): 500 μm.

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