Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

Figure 3

Reattachment of rBMSC sheets on 24-well plate (a) observed at 12 h, day 1, day 3, and day 5 by phase-contrast microscope. Cytoskeleton and nuclei double-labeled assay and rBMSC sheet observed by fluorescent microscopy in center (b) and at edge (c). Collagen type I immunofluorescence of rBMSC sheet (d). Morphology of detached rBMSC sheet (e) and one single cell as a negative control (f) observed and imaged under SEM. H&E staining of rBMSC sheet (g). Scale bar: (a), (d): 200 μm; (b), (c): 100 μm; (g): 25 μm.

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