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BioMed Research International
Volume 2017 (2017), Article ID 9475074, 9 pages
Research Article

Antithrombotic Effect and Mechanism of Radix Paeoniae Rubra

1Institute of Chinese Materia Medica, Henan University, Kaifeng 475004, China
2Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng 475004, China

Correspondence should be addressed to Wen-yi Kang

Received 25 December 2016; Revised 9 January 2017; Accepted 18 January 2017; Published 16 February 2017

Academic Editor: Veronika A. Myasoedova

Copyright © 2017 Pingyao Xie et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The compounds of Radix Paeoniae Rubra (RPR) were isolated and identified by bioassay-guided method, and antithrombotic effects and mechanism were investigated by the acute blood stasis rat model. The RPR extract was evaluated by APTT, TT, PT, and FIB assays in vitro. Results indicated that RPR extract exhibited the anticoagulant activity. In order to find active compounds, six compounds were isolated and identified, and four compounds, paeoniflorin (Pae), pentagalloylglucose (Pen), albiflorin (Ali), and protocatechuic acid (Pro), exhibited the anticoagulant activity in vitro. Therefore, the antithrombosis effects of RPR extract and four active compounds were investigated in vivo by measuring whole blood viscosity (WBV), plasma viscosity (PV), APTT, PT, TT, and FIB. Meanwhile, the levels of TXB2, 6-Keto-PGF1α, eNOS, and ET-1 were detected. Results suggested that RPR extract and four active compounds had the inhibition effect on thrombus formation, and the antithrombotic effects were associated with the regulation of vascular endothelium active substance, activating blood flow and anticoagulation effect.