Research Article

Evidence of Immunosuppressive and Th2 Immune Polarizing Effects of Antidiabetic Momordica charantia Fruit Juice

Table 1

Phytochemical compositions of plant juices.

Chemical compounds classTestMomordica charantia (part used)
Leafy stemFruit
Total juice Filtered juiceTotal juiceFiltered juice

AlkaloidsGeneral test: Dragendorff reagent
Extraction: Mayer reagent

TanninsFew drips of FeCl3, 1%+++++++++++

FlavonoidsAdding four drips of HCl 5% to 1 ml of juice++++

SaponinsFoam index (FI) of diluted aqueous decoction (positive if FI ≥ 100, meaning foam height ≥ 1 cm)++
(FI > 1 cm)

(FI < 1 cm)
++
(FI > 1 cm)

(FI < 1 cm)

TriterpenoidsLiebermann-Burchard reaction (acetic anhydride-sulfuric acid 50 : 1)+++++

MucilagesViscosity study (in absolute ethanol)++++

CoumarinsAddition of 0.5 ml of NH4OH 10%++++++

AnthraquinonesAddition of 1 ml NH4OH 25% + 1 ml NaOH+

SteroidsAcetic anhydride-chloroform + concentrated sulfuric acid++++

Cyanogenic derivatesGrignard reaction soaked paper with picric acid 5%

Chemical compounds of total and filtered juices. The phytochemical analysis was performed as described in Methods. +++, too high, ++, high, +, low, indicate the presence of the compounds in the juice; − indicates the absence of compound in the juice.