Esculetin Neutralises Cytotoxicity of t-BHP but Not of H₂O₂ on Human Leukaemia NB4 Cells

Figure 2

Metabolic activity, measured by MTT assay, of NB4 cells treated with esculetin alone or combined with oxidants. (a) Metabolic activity of NB4 cells treated with esculetin and H₂O₂. Cells (5 × 10⁵/ml) were exposed to 100 M esculetin, 1 mM H₂O₂, or both, for different times. (b) Metabolic activity of NB4 cells treated with esculetin and t-BHP. Cells (5 × 10⁵/ml) were exposed to 100 M esculetin or 100, 250, and 500 µM t-BHP separately or in combination, for the indicated times. Data are expressed as mean ± SEM of four independent experiments. Treated versus untreated cells; . Treated with esculetin plus oxidant versus esculetin alone; . Treated with esculetin plus oxidant versus oxidant alone; .

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