Figure 5: Apoptosis and necrosis of NB4 cells treated with esculetin and t-BHP. Cells (5 × 105/ml) were treated with 100 M esculetin or 100 or 500 M t-BHP separately or in combination, for different times. Cells were then stained with FITC-conjugated annexin and PI and measured by flow cytometry. One representative experiment is shown out of three. Vertical axes: propidium iodide intensity (log scale); horizontal axes: annexin-FITC intensity (log scale).